• Product NameDCP1A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total DCP1A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DCP1A.
• Target NameDCP1A
• Alternative Names    mRNA-decapping enzyme 1A; EC 3.-.-.-; Transcription factor SMIF; Smad4-interacting transcriptional co-activator; DCP1A
• Accession No. Swiss-Prot: Q9NPI6
  NCBI Gene ID: 55802
• UniprotQ9NPI6
• Gene ID55802;
• SDS-PAGE MW 75kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

Lykke-Andersen J., Mol. Cell. Biol. 22:8114-8121(2002).
Bai R.-Y., Nat. Cell Biol. 4:181-190(2002).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from Jurkat cells, using DCP1A antibody #34074.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting