• Product NameMLTK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MLTK protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human MLTK.
• Target NameMLTK
• Alternative Names    AZK; EC 2.7.11.25; MLTK; MRK-beta; mixed lineage kinase-related kinase
• Accession No. Swiss-Prot: Q9NYL2
  NCBI Gene ID: 51776
• UniprotQ9NYL2
• Gene ID51776;
• SDS-PAGE MW 91kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.

Gotoh I., J. Biol. Chem. 276:4276-4286(2001).
Bloem L.J., J. Mol. Cell. Cardiol. 33:1739-1750(2001).
Gross E.A., J. Biol. Chem. 277:13873-13882(2002).

• Western blot analysis of extracts from HeLa cells, using MLTK antibody #34083.
• Immunofluorescence analysis of HepG2 cells, using MLTK antibody #34083.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting