• Product NameNXF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NXF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human NXF1.
• Target NameNXF1
• Alternative Names    MEX67; TAP; Tip-associated protein; mRNA export factor TAP; nuclear RNA export factor 1
• Accession No. Swiss-Prot: Q9UBU9
  NCBI Gene ID: 10482
• UniprotQ9UBU9
• Gene ID10482;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export.

Braun I.C., EMBO J. 18:1953-1965(1999).
Kang Y., Genes Dev. 13:1126-1139(1999).
Bear J., Mol. Cell. Biol. 19:6306-6317(1999).

• Western blot analysis of extracts from HepG2 cells, Jurkat cells and HeLa cells, using NXF1 antibody #34096.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting