• Product NameMlx Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Mlx protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Mlx.
• Target NameMlx
• Alternative Names    BigMax protein; MAD7; MXD7; Max-like bHLHZip protein; Max-like protein X
• Accession No. Swiss-Prot: Q9UH92
  NCBI Gene ID: 6945
• UniprotQ9UH92
• Gene ID6945;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.

Billin A.N., J. Biol. Chem. 274:36344-36350(1999).
Meroni G., Oncogene 19:3266-3277(2000).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from LOVO cells, using Mlx antibody #34102.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting