• Product NameFOXD3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total FOXD3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human FOXD3.
• Target NameFOXD3
• Alternative Names    Forkhead box protein D3; HNF3/FH transcription factor genesis; FOXD3;
• Accession No. Swiss-Prot: Q9UJU5
  NCBI Gene ID: 27022
• UniprotQ9UJU5
• Gene ID27022;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.

Gregory S.G., Nature 441:315-321(2006).
Guo Y., Proc. Natl. Acad. Sci. U.S.A. 99:3663-3667(2002).
Ramsey H.C., Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from HUVEC cells and 293 cells, using FOXD3 antibody #34110.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using FOXD3 antibody #34110.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting