• Product NameMED23 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MED23 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MED23.
• Target NameMED23
• Alternative Names    Mediator of RNA polymerase II transcription subunit 23; Mediator complex subunit 23; CRSP complex subunit 3; Cofactor required for Sp1 transcriptional activation subunit 3; Transcriptional coactivator CRSP130
• Accession No. Swiss-Prot: Q9ULK4
  NCBI Gene ID: 9439
• UniprotQ9ULK4
• Gene ID9439;
• SDS-PAGE MW 150kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Required for transcriptional activation subsequent to the assembly of the preinitiation complex By similarity. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.

Wiemann S., Genome Res. 11:422-435(2001).
Mungall A.J., Nature 425:805-811(2003).
Akoulitchev S., Nature 407:102-106(2000).

• Western blot analysis of extracts from A549 cells, using MED23 antiobdy #34115.
• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MED23 antibody #34115.
• Immunofluorescence analysis of A549 cells, using MED23 antibody #34115.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting