• Product NameZNF148 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total ZNF148 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ZNF148.
• Target NameZNF148
• Alternative Names    Beta enolase repressor factor 1; G-rich box-binding protein; Transcription factor BFCOL1; Transcription factor ZBP-89; ZBP89
• Accession No. Swiss-Prot: Q9UQR1
  NCBI Gene ID: 7707
• UniprotQ9UQR1
• Gene ID7707;
• SDS-PAGE MW 89kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.

Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Boopathi E., J. Biol. Chem. 279:35242-35254(2004).
Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).

• Western blot analysis of extracts from HepG2 cells, using ZNF148 antibody #34124.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using ZNF148 antibody #34124.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting