• Product NameASF1A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ASF1A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ASF1A.
• Target NameASF1A
• Alternative Names    Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A
• Accession No. Swiss-Prot: Q9Y294
  NCBI Gene ID: 25842
• UniprotQ9Y294
• Gene ID25842;
• SDS-PAGE MW 23kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Munakata T., Genes Cells 5:221-233(2000).
Sillje H.H.W., Curr. Biol. 11:1068-1073(2001).
Lai C.-H., Genome Res. 10:703-713(2000).

• Western blot analysis of extracts from Jurkat cells, using ASF1A antibody #34126.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting