• Product NameATG4B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ATG4B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ATG4B.
• Target NameATG4B
• Alternative Names    Cysteine protease ATG4B; EC 3.4.22.-; Autophagy-related protein 4 homolog B; hAPG4B; Autophagin-1
• Accession No. Swiss-Prot: Q9Y4P1
  NCBI Gene ID: 23192
• UniprotQ9Y4P1
• Gene ID23192;
• SDS-PAGE MW 44kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Ota T.,Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from Jurkat cells, using ATG4B antibody #34136.
• Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ATG4B antibody #34136.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting