• Product Name14-3-3 ε Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total 14-3-3 ε protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human 14-3-3 ε.
• Target Name14-3-3 ε
• Alternative Names    14-3-3E; 143E; KCIP-1; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein-1
• Accession No. Swiss-Prot: P62258
  NCBI Gene ID: 7531
• UniprotP62258
• Gene ID7531;
• SDS-PAGE MW 29kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Conklin D.S., Proc. Natl. Acad. Sci. U.S.A. 92:7892-7896(1995).
Jin D.-Y., Nature 382:308-308(1996).
Chong S.S., ome Res. 6:735-741(1996).

• Western blot analysis of extracts from HepG2 cells and Jurkat cells, using 14-3-3 ε antibody #34145.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting