• Product Name14-3-3 η Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total 14-3-3 η protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human 14-3-3 η.
• Target Name14-3-3 η
• Alternative Names    14-3-3 protein eta; Protein AS1; YWHAH; YWHA1;
• Accession No. Swiss-Prot: Q04917
  NCBI Gene ID: 7533
• UniprotQ04917
• Gene ID7533;
• SDS-PAGE MW 42kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Swanson K.D., Biochim. Biophys. Acta 1216:145-148(1993).
Ichimura-Ohshima Y., J. Neurosci. Res. 31:600-605(1992).
Muratake T., Mol. Neurobiol. 11:223-230(1995).

• Western blot analysis of extracts from Jurkat cells, using 14-3-3 η antibody #34146.
• Immunofluorescence analysis of HeLa cells, using 14-3-3 η antibody #34146.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting