• Product Name14-3-3 γ Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total 14-3-3 γ protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human 14-3-3 γ.
• Target Name14-3-3 γ
• Alternative Names    1433G; 143G; KCIP-1; Protein kinase C inhibitor protein-1; YWHAG
• Accession No. Swiss-Prot: P61981
  NCBI Gene ID: 7532
• UniprotP61981
• Gene ID7532;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Autieri M.V., DNA Cell Biol. 18:555-564(1999).
Horie M., Genomics 60:241-243(1999).
Hillier L.W., Nature 424:157-164(2003).

• Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using 14-3-3 γ antibody #34147.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 γ antibody #34147.
• Immunofluorescence analysis of COS7 cells, using 14-3-3 γ antibody #34147.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting