• Product Name14-3-3 ζ Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total 14-3-3 ζ protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human 14-3-3 ζ.
• Target Name14-3-3 ζ
• Alternative Names    14-3-3 protein zeta/delta; 1433Z; 143Z; FAS; Factor activating exoenzyme S
• Accession No. Swiss-Prot: P63104
  NCBI Gene ID: 7534
• UniprotP63104
• Gene ID7534;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Zupan L.A., J. Biol. Chem. 267:8707-8710(1992).
Seluja G.A., Biochim. Biophys. Acta 1395:281-287(1998).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).

• Western blot analysis of extracts from HeLa cells, Jurkat cells and COLO cells, using 14-3-3 ζ antibody #34149.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting