• Product Name5-HT-3A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total 5-HT-3A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human 5-HT-3A.
• Target Name5-HT-3A
• Alternative Names    5-HT-3; 5-HT3R; 5-hydroxytryptamine 3 receptor; 5HT3R; HTR3
• Accession No. Swiss-Prot: P46098
  NCBI Gene ID: 3359
• UniprotP46098
• Gene ID3359;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.

Miyake A., Mol. Pharmacol. 48:407-416(1995).
Belelli D., Mol. Pharmacol. 48:1054-1062(1995).
Yamada K., Biol. Psychiatry 60:192-201(2006).

• Western blot analysis of extracts from HT-29 cells, using 5-HT-3A antibody #34152.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting