• Product NameCollagen V α1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Collagen V α1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen V α1.
• Target NameCollagen V α1
• Alternative Names    Collagen alpha-1(V) chain [Precursor]; COL5A1;
• Accession No. Swiss-Prot: P20908
  NCBI Gene ID: 1289
• UniprotP20908
• Gene ID1289;
• SDS-PAGE MW 200kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

Takahara K.,, J. Biol. Chem. 266:13124-13129(1991).
Greenspan D.S., J. Biol. Chem. 266:24727-24733(1991).
Humphray S.J., Nature 429:369-374(2004).

• Western blot analysis of extracts from HeLa cells, using Collagen V α1 antibody #34205.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using Collagen V α1 antibody #34205.
• Immunofluorescence analysis of HeLa cells, using Collagen V α1 antibody #34205.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting