• Product NameCollagen V α2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Collagen V α2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen V α2.
• Target NameCollagen V α2
• Alternative Names    Collagen alpha-2(V) chain ; CO5A2;
• Accession No. Swiss-Prot: P05997
  NCBI Gene ID: 1290
• UniprotP05997
• Gene ID1290;
• SDS-PAGE MW 145kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices By similarity.

Hillier L.W., Nature 434:724-731(2005).
Valkkila M., Matrix Biol. 20:357-366(2001).
Greenspan D.S., Gene Expr. 1:29-39(1991).

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Collagen V α2 antibody #34206.
• Immunofluorescence analysis of HepG2 cells, using Collagen V α2 antibody #34206.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting