• Product NameCollagen V α3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Collagen V α3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen V α3.
• Target NameCollagen V α3
• Alternative Names    CO5A3; collagen alpha 3;
• Accession No. Swiss-Prot: P25940
  NCBI Gene ID: 50509
• UniprotP25940
• Gene ID50509;
• SDS-PAGE MW 170kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

Imamura Y., J. Biol. Chem. 275:8749-8759(2000).
Mann K., Biol. Chem. Hoppe-Seyler 373:69-75(1992).
Molina H., Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Collagen V α3 antibody #34207.
• Immunofluorescence analysis of HeLa cells, using Collagen V α3 antibody #34207.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting