• Product NameCollagen XII α1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Collagen XII α1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Collagen XII α1.
• Target NameCollagen XII α1
• Alternative Names    Collagen alpha-1(XII) chain; COCA1;
• Accession No. Swiss-Prot: Q99715
  NCBI Gene ID: 1303
• UniprotQ99715
• Gene ID1303;
• SDS-PAGE MW 333kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix By similarity.

Gerecke D.R., Genomics 41:236-242(1997).
Mungall A.J., Nature 425:805-811(2003).
Chiquet M., Eur. J. Biochem. 257:362-371(1998).

• Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Collagen XII α1 antibody #34213.
• Immunofluorescence analysis of COS-7 cells, using Collagen XII α1 antibody #34213.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting