• Product NameCytochrome P450 24A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Cytochrome P450 24A1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Cytochrome P450 24A1.
• Target NameCytochrome P450 24A1
• Alternative Names    1,25-dihydroxyvitamin D; 24- hydroxylase; 24-OHase; 24-hydroxylase
• Accession No. Swiss-Prot: Q07973
  NCBI Gene ID: 1591
• UniprotQ07973
• Gene ID1591;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.

Chen K.-S., Proc. Natl. Acad. Sci. U.S.A. 90:4543-4547(1993).
Deloukas P., Nature 414:865-871(2001).
Chen K.-S., Biochim. Biophys. Acta 1263:1-9(1995).

• Western blot analysis of extracts from HUVEC cells, using Cytochrome P450 24A1 antibody #34229.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting