• Product NameCytochrome P450 27A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Cytochrome P450 27A1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Cytochrome P450 27A1.
• Target NameCytochrome P450 27A1
• Alternative Names    25-hydroxylase; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; CP27A
• Accession No. Swiss-Prot: Q02318
  NCBI Gene ID: 1593
• UniprotQ02318
• Gene ID1593;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.

Cali J.J., J. Biol. Chem. 266:7774-7778(1991).
Guo Y.-D., Proc. Natl. Acad. Sci. U.S.A. 90:8668-8672(1993).
Leitersdorf E., J. Clin. Invest. 91:2488-2496(1993).

• Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody #34231.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting