• Product NameCytochrome P450 2C8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Cytochrome P450 2C8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Cytochrome P450 2C8.
• Target NameCytochrome P450 2C8
• Alternative Names    Cytochrome P450 2C8; EC 1.14.14.1; CYPIIC8; P450 form 1; P450 MP-12/MP-20
• Accession No. Swiss-Prot: P10632
  NCBI Gene ID: 1558
• UniprotP10632
• Gene ID1558;
• SDS-PAGE MW 56kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it generates only 14,15- and 11,12-cis-epoxyeicosatrienoic acids. It is the principal enzyme responsible for the metabolism the anti-cancer drug paclitaxel (taxol).

Romkes M., Biochemistry 30:3247-3255(1991).
Ged C., Biochim. Biophys. Acta 1088:433-435(1991).
Zeldin D.C., Arch. Biochem. Biophys. 322:76-86(1995).

• Western blot analysis of extracts from HuvEc cells, using Cytochrome P450 2C8 antiobdy #34235.
• Immunohistochemistry analysis of paraffin-embedded human testis tissue, using Cytochrome P450 2C8 antibody #34235.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting