• Product NameCytochrome P450 39A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Cytochrome P450 39A1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Cytochrome P450 39A1.
• Target NameCytochrome P450 39A1
• Alternative Names    Cytochrome P450 39A1; EC 1.14.13.99; 24-hydroxycholesterol 7-alpha-hydroxylase; Oxysterol 7-alpha-hydroxylase; Hcyp39a1
• Accession No. Swiss-Prot: Q9NYL5
  NCBI Gene ID: 51302
• UniprotQ9NYL5
• Gene ID51302;
• SDS-PAGE MW 54kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.

Li-Hawkins J., . Biol. Chem. 275:16543-16549(2000).
The MGC Project Team, ome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from LOVO cells and HUVEC cells, using Cytochrome P450 39A1 antibody #34242.
• Immunofluorescence analysis of A549 cells, using Cytochrome P450 39A1 antibody #34242.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting