• Product NameCYB5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CYB5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CYB5.
• Target NameCYB5
• Alternative Names    Cytochrome b5; CYB5A; CYB5;
• Accession No. Swiss-Prot: P00167
  NCBI Gene ID: 1528
• UniprotP00167
• Gene ID1528;
• SDS-PAGE MW 15kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Yoo M., Biochem. Biophys. Res. Commun. 156:576-580(1988).
Li X.R., Biochem. Biophys. Res. Commun. 209:894-900(1995).
Giordano S.J., Biochem. Biophys. Res. Commun. 178:38-44(1991).

• Western blot analysis of extracts from A549 cells, using CYB5 antibody #34264.
• Immunofluorescence analysis of NIH/3T3 cells, using CYB5 antibody #34264.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting