• Product NameDNL4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DNL4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DNL4.
• Target NameDNL4
• Alternative Names    DNA ligase 4; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
• Accession No. Swiss-Prot: P49917
  NCBI Gene ID: 3981
• UniprotP49917
• Gene ID3981;
• SDS-PAGE MW 103kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Wei Y.-F., Mol. Cell. Biol. 15:3206-3216(1995).
Dunham A., Nature 428:522-528(2004).
Robins P., J. Biol. Chem. 271:24257-24261(1996).

• Western blot analysis of extracts from Jurkat cells, using DNL4 antibody #34266.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting