• Product NameLEG8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total LEG8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human LEG8.
• Target NameLEG8
• Alternative Names    Galectin-8; Gal-8; Prostate carcinoma tumor antigen 1; PCTA-1; Po66 carbohydrate-binding protein
• Accession No. Swiss-Prot: O00214
  NCBI Gene ID: 3964
• UniprotO00214
• Gene ID3964;
• SDS-PAGE MW 36kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.

Su Z.-Z., Proc. Natl. Acad. Sci. U.S.A. 93:7252-7257(1996).
Hadari Y.R., Trends Glycosci. Glycotechnol. 9:103-112(1997).
Gopalkrishnan R.V., Oncogene 19:4405-4416(2000).

• Western blot analysis of extracts from 3T3 cells, using LEG8 antibody #34284.
• Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using LEG8 antibody #34284.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting