• Product NamePARP2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PARP2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PARP2.
• Target NamePARP2
• Alternative Names    Poly [ADP-ribose] polymerase 2; PARP-2; NAD(+) ADP-ribosyltransferase 2; Poly[ADP-ribose] synthetase 2; pADPRT-2
• Accession No. Swiss-Prot: Q9UGN5
  NCBI Gene ID: 10038
• UniprotQ9UGN5
• Gene ID10038;
• SDS-PAGE MW 75kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

Sugano S., Nat. Genet. 36:40-45(2004).
de Murcia G.M., J. Biol. Chem. 277:23028-23036(2002).
Bennett K.L., Submitted (OCT-2008).

• Western blot analysis of extracts from K562 and HuvEc cells, using PARP2 antibody #34292.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting