• Product NamePSMD11 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PSMD11 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PSMD11.
• Target NamePSMD11
• Alternative Names    26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit p44.5; 26S proteasome regulatory subunit S9; PSD11;
• Accession No. Swiss-Prot: O00231
  NCBI Gene ID: 5717
• UniprotO00231
• Gene ID5717;
• SDS-PAGE MW 42kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Hoffman L., FEBS Lett. 404:179-184(1997).
Saito A., Gene 203:241-250(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from 293 cells and HepG2 cells, using PSMD11 antibody #34307.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting