• Product NameACOT4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ACOT4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ACOT4.
• Target NameACOT4
• Alternative Names    acyl-CoA thioesterase 4; acyl-coenzyme A thioesterase 4; EC 3.1.2.2; peroxisomal acyl coenzyme A thioester hydrolase Ib; peroxisomal long-chain acyl-CoA thioesterase Ib
• Accession No. Swiss-Prot: Q8N9L9
  NCBI Gene ID: 122970
• UniprotQ8N9L9
• Gene ID122970;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH By similarity. Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs.

Ota T., Nat. Genet. 36:40-45(2004).
Li W.B., Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from HeLa cells and COLO cells, using ACOT4 antibody #34382.
• Immunofluorescence analysis of MCF-7 cells, using ACOT4 antibody #34382.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting