• Product NameAKR1C2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total AKR1C2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human AKR1C2.
• Target NameAKR1C2
• Alternative Names    3-alpha-HSD3; AK1C2; AKR1C-pseudo; aldo-keto reductase family 1 member C2; BABP
• Accession No. Swiss-Prot: P52895
  NCBI Gene ID: 1646
• UniprotP52895
• Gene ID1646;
• SDS-PAGE MW 37kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability.

Qin K.-N., J. Steroid Biochem. Mol. Biol. 46:673-679(1993).
Ciaccio P.J., Biochim. Biophys. Acta 1186:129-132(1994).
Qin K.-N., Gene 149:357-361(1994).

• Western blot analysis of extracts from COLO cells, using AKR1C2 antibody #34400.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting