• Product NameAPOA5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total APOA5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human APOA5.
• Target NameAPOA5
• Alternative Names    Apolipoprotein A-V; Apo-AV; ApoA-V; Apolipoprotein A5; Regeneration-associated protein 3
• Accession No. Swiss-Prot: Q6Q788
  NCBI Gene ID: 116519
• UniprotQ6Q788
• Gene ID116519;
• SDS-PAGE MW 41kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) By similarity. Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.

van Der Vliet H.N., J. Biol. Chem. 276:44512-44520(2001) [PubMed: 11577099].
Beckstead J.A., Biochemistry 42:9416-9423(2003) [PubMed: 12899628].
Fullerton S.M., Hum. Genet. 115:36-56(2004) [PubMed: 15108119].

• Western blot analysis of extracts from 293 cells, using APOA5 antibody #34429.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting