• Product NameATP5L2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ATP5L2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ATP5L2.
• Target NameATP5L2
• Alternative Names    ATP synthase subunit g 2; mitochondrial; ATPase subunit g 2; ATP5K2;
• Accession No. Swiss-Prot: Q7Z4Y8
  NCBI Gene ID: 267020
• UniprotQ7Z4Y8
• Gene ID267020;
• SDS-PAGE MW 20kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane By similarity.

Lin W., Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Western blot analysis of extracts from A549 cells, using ATP5L2 antibody #34454.
• Immunofluorescence analysis of A549 cells, using ATP5L2 antibody #34454.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting