• Product NameDDX54 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DDX54 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DDX54.
• Target NameDDX54
• Alternative Names    ATP-dependent RNA helicase DDX54; EC=3.6.4.13; ATP-dependent RNA helicase DP97; DEAD box RNA helicase 97 kDa; DEAD box protein 54
• Accession No. Swiss-Prot: Q8TDD1
  NCBI Gene ID: 79039
• UniprotQ8TDD1
• Gene ID79039;
• SDS-PAGE MW 98kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.

Scherer S.E., Nature 440:346-351(2006) [PubMed: 16541075].
Rajendran R.R., J. Biol. Chem. 278:4628-4638(2003) [PubMed: 12466272].
Scherl A.,Mol. Biol. Cell 13:4100-4109(2002) [PubMed: 12429849].

• Western blot analysis of extracts from CoLo cells, 293 cells and JurKat cells, using DDX54 antibody #34469.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting