• Product NameBCL2L12 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total BCL2L12 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human BCL2L12.
• Target NameBCL2L12
• Alternative Names    Bcl-2-like protein 12; Bcl2-L-12; Bcl-2-related proline-rich protein;
• Accession No. Swiss-Prot: Q9HB09
  NCBI Gene ID: 83596
• UniprotQ9HB09
• Gene ID83596;
• SDS-PAGE MW 34kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

This gene encodes a member of a family of proteins containing a Bcl-2 homology domain 2 (BH2). The encoded protein is an anti-apoptotic factor that acts as an inhibitor of caspases 3 and 7 in the cytoplasm. In the nucleus, it binds to the p53 tumor suppressor protein, preventing its association with target genes. Overexpression of this gene has been detected in a number of different cancers. There is a pseudogene for this gene on chromosome 3. Alternative splicing results in multiple transcript variants.

Scorilas A., Genomics 72:217-221(2001) [PubMed: 11401436].
Hillman R.T., Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258].
Olsen J.V., Cell 127:635-648(2006) [PubMed: 17081983].

• Western blot analysis of extracts from CoLo cells, using BCL2L12 antibody #34482.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting