• Product NameGCNT3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total GCNT3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human GCNT3.
• Target NameGCNT3
• Alternative Names    Beta-1; 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase 3; EC 2.4.1.102; EC 2.4.1.150
• Accession No. Swiss-Prot: O95395
  NCBI Gene ID: 9245
• UniprotO95395
• Gene ID9245;
• SDS-PAGE MW 51kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.

Ishibashi Y., Glycoconj. J. 22:53-62(2005).
Huang M.-C., Oncogene 25:3267-3276(2006).
Tan S., Am. J. Respir. Cell Mol. Biol. 36:737-745(2007).

• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using GCNT3 antibody #34483.
• Immunofluorescence analysis of A549 cells, using GCNT3 antibody #34483.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting