• Product NamePNPLA8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PNPLA8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PNPLA8.
• Target NamePNPLA8
• Alternative Names    calcium-independent phospholipase A2-gamma; EC 3.1.1.5; intracellular membrane-associated calcium-independent phospholipase A2 gamma; iPLA2-gamma; patatin-like phospholipase domain-containing protein 8
• Accession No. Swiss-Prot: Q9NP80
  NCBI Gene ID: 50640
• UniprotQ9NP80
• Gene ID50640;
• SDS-PAGE MW 88kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids.

Tanaka H., Biochem. Biophys. Res. Commun. 272:320-326(2000).
Mancuso D.J., J. Biol. Chem. 275:9937-9945(2000).
Hillier L.W., Nature 424:157-164(2003).

• Western blot analysis of extracts from HeLa cells, A549 cells and HUVEC cells, using PNPLA8 antibody #34518.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting