• Product NameCHST8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CHST8 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CHST8.
• Target NameCHST8
• Alternative Names    carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8; carbohydrate sulfotransferase 8; EC 2.8.2.-; GalNAc-4-O-sulfotransferase 1; GalNAc-4-ST1
• Accession No. Swiss-Prot: Q9H2A9
  NCBI Gene ID: 64377
• UniprotQ9H2A9
• Gene ID64377;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin.

Okuda T., J. Biol. Chem. 275:40605-40613(2000).
Hiraoka N., Glycobiology 11:495-504(2001).
Barret A., J. Biol. Chem. 280:10516-10523(2005).

• Western blot analysis of extracts from K562 cells, using CHST8 antibody #34536.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting