• Product NameCDC42EP3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CDC42EP3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human CDC42EP3.
• Target NameCDC42EP3
• Alternative Names    Binder of Rho GTPases 2; BORG2; CDC42 effector protein (Rho GTPase binding) 3; Cdc42 effector protein 3; CDC42EP3
• Accession No. Swiss-Prot: Q9UKI2
  NCBI Gene ID: 10602
• UniprotQ9UKI2
• Gene ID10602;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts.

Joberty G., Mol. Cell. Biol. 19:6585-6597(1999).
Hirsch D.S., J. Biol. Chem. 276:875-883(2001).
Wiemann S., Genome Res. 11:422-435(2001).

• Western blot analysis of extracts from K562 cells, using CDC42EP3 antibody #34557.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting