• Product NameCHSY1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CHSY1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CHSY1.
• Target NameCHSY1
• Alternative Names    carbohydrate (chondroitin) synthase 1; chondroitin glucuronyltransferase II; chondroitin synthase; CHSS1; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1
• Accession No. Swiss-Prot: Q86X52
  NCBI Gene ID: 22856
• UniprotQ86X52
• Gene ID22856;
• SDS-PAGE MW 91kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling.

Kitagawa H., J. Biol. Chem. 276:38721-38726(2001).
Nagase T., DNA Res. 6:63-70(1999).
Clark H.F., Genome Res. 13:2265-2270(2003).

• Western blot analysis of extracts from HepG2 cells and K562 cells, using CHSY1 antibody #34588.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CHSY1 antibody #34588.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting