• Product NameCHSS2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CHSS2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CHSS2.
• Target NameCHSS2
• Alternative Names    CHSS2; DKFZP434E0423; EC 2.4.1.175; EC 2.4.1.226;
• Accession No. Swiss-Prot: Q8IZ52
  NCBI Gene ID: 79586
• UniprotQ8IZ52
• Gene ID79586;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PARK2 transport into the mitochondria. In collaboration with PARK2, isoform 2 may enhance cell viability and protect cells from oxidative stress.

Kitagawa H., J. Biol. Chem. 278:23666-23671(2003).
Yada T., J. Biol. Chem. 278:30235-30247(2003).
Clark H.F., Genome Res. 13:2265-2270(2003)

• Western blot analysis of extracts from LOVO cells and K562 cells, using CHSS2 antibody #34589.
• Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using CHSS2 antibody #34589.
• Immunofluorescence analysis of MCF-7 cells, using CHSS2 antibody #34589.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting