• Product NameDHRS2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DHRS2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DHRS2.
• Target NameDHRS2
• Alternative Names    dehydrogenase/reductase (SDR family) member 2; dehydrogenase/reductase SDR family member 2; HEP27; protein D; SDR25C1
• Accession No. Swiss-Prot: Q13268
  NCBI Gene ID: 10202
• UniprotQ13268
• Gene ID10202;
• SDS-PAGE MW 27kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.

Gabrielli F., Eur. J. Biochem. 232:473-477(1995).
Pellegrini S., Biochim. Biophys. Acta 1574:215-222(2002).
Suzuki Y., Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from COLO cells, using DHRS2 antibody #34643.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting