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LC3 antibody

21400
Review: comment rank 5 pdf
  Catalog No. Package Size Price
21400-1 50ul $189.00
21400-2 100ul $279.00

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Description
  • Product Name LC3 antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total LC3 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa. 112-116 (V-Y-A-S-Q) derived from Human LC3.
  • Target Name LC3
  • Alternative Names LC3A; ATG8E; MAP1ALC3; MAP1BLC3;
  • Accession No. Swiss-Prot: Q9H492
    NCBI Protein: NP_115903.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 19kd
Western blotting: 1:500~1:1000

Images
  • LC3 antibody - SAB | Signalway Antibody

    Western blot analysis of extract from K-562, Hela, JK and 293 cells using LC3 Antibody #21400

Background
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
    Please let us know if you have published research using #21400 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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