• Product NameMARCH4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MARCH4 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MARCH4.
• Target NameMARCH4
• Alternative Names    E3 ubiquitin-protein ligase MARCH4 ; EC 6.3.2.-; Membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; MARCH-IV
• Accession No. Swiss-Prot: Q9P2E8
  NCBI Gene ID: 57574
• UniprotQ9P2E8
• Gene ID57574;
• SDS-PAGE MW 46kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100

E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Nagase T., DNA Res. 7:65-73(2000).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Bartee E., J. Virol. 78:1109-1120(2004).

• Immunohistochemistry analysis of paraffin-embedded human brain tissue using MARCH4 antibody #34661.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting