• Product NameRAD18 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RAD18 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RAD18.
• Target NameRAD18
• Alternative Names    E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; postreplication repair protein hRAD18p; Postreplication repair protein RAD18
• Accession No. Swiss-Prot: Q9NS91
  NCBI Gene ID: 56852
• UniprotQ9NS91
• Gene ID56852;
• SDS-PAGE MW 56kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Tateishi S., Proc. Natl. Acad. Sci. U.S.A. 97:7927-7932(2000).
Xin H., Nucleic Acids Res. 28:2847-2854(2000).
Muzny D.M., Nature 440:1194-1198(2006).

• Western blot analysis of extracts from LOVO cells, HepG2 cells and K562 cells, using RAD18 antibody #34662.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting