• Product NameFUT3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total FUT3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human FUT3.
• Target NameFUT3
• Alternative Names    Galactoside 3(4)-L-fucosyltransferase; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; FucT-III
• Accession No. Swiss-Prot: P21217
  NCBI Gene ID: 2525
• UniprotP21217
• Gene ID2525;
• SDS-PAGE MW 42kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

Kukowska-Latallo J.F., Genes Dev. 4:1288-1303(1990).
Cameron H.S., J. Biol. Chem. 270:20112-20122(1995).
Rahim I., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.

• Western blot analysis of extracts from K562 cells and Jurkat cells, using MRPS7 antibody #34693.
• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MRPS7 antibody #34693.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting