• Product NameRAD Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total RAD protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human RAD.
• Target NameRAD
• Alternative Names    GTP-binding protein RAD; RAD; RAD1; RAS associated with diabetes;
• Accession No. Swiss-Prot: P55042
  NCBI Gene ID: 6236
• UniprotP55042
• Gene ID6236;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane By similarity. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.

Reynet C., Science 262:1441-1444(1993).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Moyers J.S., J. Biol. Chem. 272:11832-11839(1997).

• Western blot analysis of extracts from HeLa cells, HepG2 cells and MCF-7 cells, using RAD antibody #34723.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using RAD antibody #34723.
• Immunofluorescence analysis of A549 cells, using RAD antibody #34723.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting