• Product NameHLA-DOA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HLA-DOA protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human HLA-DOA.
• Target NameHLA-DOA
• Alternative Names    HLA class II histocompatibility antigen; DO alpha chain [Precursor]; MHC class II antigen DOA; MHC DZ alpha; MHC DN-alpha
• Accession No. Swiss-Prot: P06340
  NCBI Gene ID: 3111
• UniprotP06340
• Gene ID3111;
• SDS-PAGE MW 34kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

Trowsdale J., EMBO J. 4:2231-2237(1985).
Jonsson A.-K., Immunogenetics 29:411-413(1989).
Young J.A., Immunogenetics 31:386-388(1990).

• Western blot analysis of extracts from COLO cells, using HLA-DOA antibody #34735.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting