• Product NameHLA-DOB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HLA-DOB protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human HLA-DOB.
• Target NameHLA-DOB
• Alternative Names    HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB;
• Accession No. Swiss-Prot: P13765
  NCBI Gene ID: 3112
• UniprotP13765
• Gene ID3112;
• SDS-PAGE MW 31kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

Tonnelle C., EMBO J. 4:2839-2847(1985).
Jonsson A.-K., Immunogenetics 29:411-413(1989).
Beck S., J. Mol. Biol. 228:433-441(1992).

• Western blot analysis of extracts from 293 cell, using HLA-DOB antibody #34736.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting