• Product NameLIPI Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total LIPI protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human LIPI.
• Target NameLIPI
• Alternative Names    EC 3.1.1.-; lipase member I; LPD lipase; LPDL; membrane-associated phosphatidic acid-selective phospholipase A1-beta
• Accession No. Swiss-Prot: Q6XZB0
  NCBI Gene ID: 149998
• UniprotQ6XZB0
• Gene ID149998;
• SDS-PAGE MW 53kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).

Hiramatsu T., J. Biol. Chem. 278:49438-49447(2003).
Wen X.-Y., Hum. Mol. Genet. 12:1131-1143(2003).
Hattori M., Nature 405:311-319(2000).

• Western blot analysis of extracts from COLO cells, using LIPI antibody #34760.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting