• Product NameMED18 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MED18 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MED18.
• Target NameMED18
• Alternative Names    FLJ20045; mediator 18; mediator of RNA polymerase II transcription 18; p28b;
• Accession No. Swiss-Prot: Q9BUE0
  NCBI Gene ID: 54797
• UniprotQ9BUE0
• Gene ID54797;
• SDS-PAGE MW 24kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Furumoto T., Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Gregory S.G., Nature 441:315-321(2006).

• Western blot analysis of extracts from Jurkat cells and COLO cells, using MED18 antibody #34777.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting