• Product NameMBTPS2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total MBTPS2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MBTPS2.
• Target NameMBTPS2
• Alternative Names    Membrane-bound transcription factor site-2 protease; EC 3.4.24.85; S2P endopeptidase; Site-2 protease; Sterol regulatory element-binding proteins intramembrane protease
• Accession No. Swiss-Prot: O43462
  NCBI Gene ID: 51360
• UniprotO43462
• Gene ID51360;
• SDS-PAGE MW 58kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  
  Immunofluorescence: 1:100~1:500

Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.

Rawson R.B., Mol. Cell 1:47-57(1997).
Ye J., Proc. Natl. Acad. Sci. U.S.A. 97:5123-5128(2000).

• Immunofluorescence analysis of A549 cells, using MBTPS2 antibody #34784.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting